Isocratic vs Gradient
In isocratic mode a constant single solvent or constant mixture of solvents are used in analysis where as in gradient mode the composition of solvents is changed constantly while performing analysis for enhancing the separation efficiency i.e to get faster elution of sample from mixture.
In isocratic mode a constant single solvent or constant mixture of solvents are used in analysis where as in gradient mode the composition of solvents is changed constantly while performing analysis for enhancing the separation efficiency i.e to get faster elution of sample from mixture.